CYTOTOXIC EFFECTS OF NOVEL SYNTHETIC CANNABINOID, PARENT COMPOUND, THJ-018, ON SH-SY5Y NEUROBLASTOMA CELLS

Sakenov, R. (2019) CYTOTOXIC EFFECTS OF NOVEL SYNTHETIC CANNABINOID, PARENT COMPOUND, THJ-018, ON SH-SY5Y NEUROBLASTOMA CELLS. Scientific-Practical Journal of Medicine, "Vestnik KazNMU" (1). ISSN 2524 - 0692

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CYTOTOXIC EFFECTS OF NOVEL SYNTHETIC CANNABINOID, PARENT COMPOUND, THJ-018, ON SH-SY5Y NEUROBLASTOMA CELLS.pdf

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Abstract

Novel synthetic cannabinoids pose serious health risks to the society. One of the most significant concerns is constant appearance of new synthetic cannabinoids on grey markets. These substances cause numerous toxic effects in human body, ranging from neurotoxic effects to cardiovascular toxicity and acute kidney injury as well as addiction and withdrawal syndromes. Thus, the aim of this study was to investigate cytotoxic effects of novel synthetic cannabinoids, THJ-018, on human neuronal cell line. The work reports the cytotoxicity of novel synthetic cannabinoid, THJ-018, on neuroblastoma, SH-SY5Y cell line, which has not yet been published in the scientific literature. To study cytotoxicity of novel synthetic cannabinoids, SH-SY5Y neuroblastoma cell line was chosen to model cytotoxic effects of THJ-018 on neuronal cells in concentration of 5x10^4 cells/ml in vitro. THJ-018, dissolved in DMSO, was tested in concentrations from 1.0 to 100.0 μМ during 24 hours of exposure. To investigate cell viability, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used. To count the number and percentage of viable cells, trypan blue (TB) cell counting assay, and Neubauer chamber were employed. THJ-018 did not affect the viability of SH-SY5Y cells after 24h exposure in cell culture in vitro in the concentration range from 1.0 to 75.0 μМ. The results of MTT and TB assays showed that THJ-018 had no statistically significant cytotoxic effect on SH-SY5Y cells in terms of formazan production by mitochondria and percentage of viable cells in cell culture in vitro at these concentrations. However, at THJ-018 concentration of 100.0 μМ, production of formazan decreased from 100% to 71.5%± 11.8% if compared to negative control. The results of TB assay after 24h exposure to THJ-018 were in a good accordance with the previous results of the MTT assay. THJ-018 showed no cytotoxic effects on cellular viability in terms of the percentage of viable cells at concentrations from 1.0 to 75.0 μМ. But, at the concentrations from 75.0 to 100.0 μМ, percentage of viable SH-SY5Y cells in cell culture in vitro dropped from 100% to 67.3±15.6% and to 49.5±16.6% correspondingly, if compared to negative control. The results of the study confirmed previously published reports that parent compounds of synthetic cannabinoids are less toxic than their secondary metabolites and smoke products in the concentration range from 1.0 to 100.0 μМ. However, at higher concentrations, cytotoxic effects become evident in terms of general cellular viability of mitochondria and percentage of viable cells. The results of the work contribute to further understanding of mechanisms of cytotoxicity of synthetic cannabinoids in vitro.

Item Type: Article
Uncontrolled Keywords: THJ-018, synthetic cannabinoids, neurotoxicity, cytotoxicity, forensic toxicology, SH-SY5Y cells
Subjects: R Medicine > R Medicine (General)
Divisions: Научно-практический журнал "Вестник КазНМУ" > Выпуск №4 2016 год
Depositing User: Mr Askhat Zhakaev
Date Deposited: 04 Jul 2019 10:26
Last Modified: 04 Jul 2019 10:26
URI: http://repository.kaznmu.kz/id/eprint/15576

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